BCOP – Alternative Ocular Irritation Test

BCOP – Bovine Cornea Opacity/Permeability Test – Eye Irritation Testing

BCOP Ocular Irritation Testing-In Vitro Toxicology
BCOP Ocular Irritation Testing-In Vitro Toxicology

Just What is the BCOP?

The BCOP (Bovine Corneal Opacity and Permeability) test is a mechanistic in vitro eye irritation assay developed by Gautheron et al. (1992), that utilizes excised bovine corneas, obtained as a by-product from abattoirs.

The BCOP is used to evaluate the potential ocular irritancy of a test material. Irritation caused by the topically applied test material is quantitatively measured by changes in opacity and permeability. Corneal opacity can be caused by protein denaturation or the swelling of the stroma, while corneal permeability indicates the loss in barrier function and cell-to-cell membrane junctions of the corneal epithelia.

Can the BCOP be used in determining Eye Irritation Potential for Regulatory Purposes?

The BCOP is a quick and cost-effective assay to determine ocular irritation.  The BCOP is accepted as an alternative to the Draize Rabbit Eye Test by several regulatory agencies.  In the US and in Europe, the BCOP assay is approved for identifying corrosive substances and those chemicals not requiring classification according to UN GHS.

• OECD Test Guideline (TG 437) was adopted  in 2009 and revised in 2013.

• US EPA accepts the BCOP test as part of a tiered testing strategy for anti-microbial cleaning products.

Get More Info on the BCOP…

For a Quotation, please feel free to contact Client Services. Discounted pricing is available for the placement of multiple studies.

Advancing In Vitro & Alternative Toxicology

The BCOP can be used in combination with other in vitro and alternative assays to gain a better sense of eye irritation potential without using live rabbits. MB Research has extensive experience in coordinating integrated testing strategies. (See ROBatt)

Need other in vitro toxicology testing?

Cosmetics and Personal Care Products Safety Testing

Cosmetics Safety Testing/Personal Care Products Safety Testing
Cosmetics Safety Testing/Personal Care Products Safety Testing “Woman at her toilette”, Henri de Toulouse-Lautrec, 1889.

Safety Testing Capabilities for Cosmetics and Personal Care Products

In order to address the need for alternative to animal toxicology testing by the cosmetic and personal care products industries, MB Research offers a wide array of in vitro toxicology assays used to assure the safety of their products.

Several assays are available for determining the potential of eye and skin irritation. For harsher ingredients, assays are also available for in vitro corrosivity screening. Many of these test methods are based on the use of 3D human tissue constructs that mimic the epithelia layer of skin or the cornea. Some of these 3D tissues are full-thickness that include an endothelial layer of fibroblasts.

Other key test methods include cytotoxicity tests, phototoxicity screening, dermal sensization (skin allergy screening) and genotoxicity(Ames).

Check out In Vitro Toxicology Testing, for more info.

In Vitro Toxicology Testing of Personal Care & Cosmetic Products

In Vitro Toxicology Testing for Personal Care and Cosmetic Products. MB Research Labs offers a wide variety of in vitro and alternative toxicology assays to ensure the safety of the ingredients used in personal care and cosmetic products.

See more info about in vitro toxicology testing.

I Love In Vitro Toxicology
I Love In Vitro Toxicology

Advancing Predictive Toxicology Methods using QSAR & Read-Across

Here is another interesting webinar provided by ICCVAM regarding the use of Quantitative SAR models and Read-Across Techniques that may aid in the development of predictive in vitro toxicology testing methods.

Areas of Impact. In Vitro Toxicology Testing.
Areas of Impact. In Vitro Toxicology Testing.

Registration is still open!
ICCVAM Communities of Practice Webinar 2016 – Fundamentals of Using Quantitative Structure–Activity Relationship Models and Read-across Techniques in Predictive Toxicology
January 26, 2016 — 1:00-2:30 p.m. Eastern Standard Time

Many commercial and environmental chemicals lack toxicity data necessary for users and risk assessors to make informed decisions about their potential health effects.  Computational methods use data about structure, properties, and toxicity from tested chemicals to make predictions about the characteristics of untested chemicals.  These include quantitative structure–activity relationship models, which predict the activities of chemicals with unknown properties by relating them to properties of known chemicals, and read-across, which uses toxicity data from a known (source) chemical to predict toxicity for another (target) chemical, usually but not always on the basis of structural similarity.  Predictions made using these methods about toxicity of untested chemicals can help set priorities for future in vitro or in vivo testing, ensuring that the most important hazards are characterized first and that testing resources are used efficiently.

More information regarding In Vitro Toxicology Testing.

New Year – New Path – In Vitro Toxicology Testing

New Year - New Path - In Vitro Toxicology
New Year – New Path – In Vitro Toxicology

“Do not go where the path may lead, go instead where there is no path and leave a trail.”
Ralph Waldo Emerson

In Vitro Toxicology Testing

In Vitro Toxicology Testing is more widely accepted than before. More in vitro and alternative testing methods are being validated and accepted by regulatory agencies at an increasingly faster pace. Technology is rapidly advancing new approaches to replacing the use of animals in toxicological studies.

MB Research Labs is a pioneer in the use and development of in vitro toxicology testing. Our scientists have been working diligently on developing new in vitro test methods such as, 3D human tissue constructs, co-culture systems, and kinetic tissue culture methods.

Want to find out more about MB Research Labs?

In Vitro Test Methods

MB Research Labs offers many in vitro toxicology assays used in the chemical, cosmetic and pharmaceutical industries to assess toxicity. Want more info on our capabilities, Download Now….

Partial List of In Vitro Toxicology Testing Capabilities:

  • Eye and Skin Irritation Screening
  • Eye and Skin Corrosivity Testing
  • Genotoxicity – Ames Test
  • Phototoxicity Screening – 3T3 NRU PT and 3D Human Tissues
  • Acute Dermal Sensitization Assays – hCLAT, DPRA, IVSA, Dendritic Cell Activation (Flow Cytometry)

What if there isn’t an Alternative Available?

MB Research Labs may be able to help there too. With over 40 years of Acute Toxicology Testing experience, MB Research has standard protocols that meet pre-clinical testing requirements for EPA, FDA, OECD, GHS and other guidelines. More…

Shipping Corrosive Materials?

in vitro Corrosivity testing - in vitro toxicology
in vitro Corrosivity testing – in vitro toxicology

What is Corrositex®?

Corrositex® is an in vitro method used to determine the dermal corrosive potential of chemicals and chemical mixtures. Corrositex® has been designed as a replacement for the dermal corrosivity rabbit test based upon proprietary bio-membrane and chemical detection technology developed by In Vitro International.

Why Use Corrositex®?

time-buttonTime Savings: Corrositex® testing can provide a Packing Group determination in a matter of minutes and no more than 4 hours, unlike animal testing which can take 2 – 4 weeks.

accuracy-buttonAccuracy: More accurate than pH testing and is packing group specific.

saving-costCost Savings: Reduced shipping charges, additional cost savings workplace safety and MSDS development.


Why Should I Have Corrositex Performed at MB?

  • Unbiased 2nd Party Test Results in a Report Format
  • Reputable Contract Lab with over 40 years of Regulatory Testing Experience
  • We worry about the details. Less Hassle and Effort on your part.

Can I get a discount for having multiple products tested?

Absolutely, we offer aggressive discounting for multiple tests performed at the same time.

If my test material doesn’t cause a change in color in the Corrositex® chemical detection fluid, what then?

Chemicals are prescreened for compatibility with Corrositex® by directly applying the test chemical or chemical mixture to the detection fluid; if a color change is not induced, then the test chemical or chemical mixture does not qualify for testing with Corrositex®. Contact MB Research, we can offer you more testing options.

For more info about Corrositex Testing, check out www.corrositex.com!

MB Research Labs – Your In Vitro Toxicologist

In Vitro Toxicology Testing with 3D Human Tissue Constructs

in vitro toxicology - tissue culture
in vitro toxicology – tissue culture
MB Research Labs – Advancing In Vitro/Alternative Test Methods.

Three dimensional human tissue constructs have proven to be very effective screening tools for irritation and corrosivity testing for the cosmetic, petrochemical, and pharmaceutical industries. Tissue constructs have been utilized for many years for ocular and dermal toxicity screening at MB Research Labs

Other tissue types (buccal, brochial, vaginal, dermal, ocular, tanned epidermis) that exhibit in vivo-like morphological and growth characteristics are available from various vendors. MB Research frequently works closely with these manufacturers to develop innovative testing protocols that fit our clients’ needs.

More and more interest in the utility of these 3D tissue models has increased especially since their use reduces the number of animals used in Toxicology testing.

MatTek EpiDerm and EpiOcular
SkinEthic Reconstituted Human Epidermal and Epithelial Models
CellSystems EpiCS® – Reconstructed Human Epidermis (RhE) models
InSphero – 3D Tissue Spheroids

Others may be available upon request.

More on In Vitro Toxicology Testing…

In Vitro Sensitization – First Steps

Sensitization testing for household and consumer products has traditionally been performed using in vivo study models with guinea pigs and mice.

MB Research now offers an Integrated Testing Strategy (ITS) of in vitro toxicology tests that follows a specific Adverse Outcome Pathway for acute dermal sensitization.

sensitization adverse outcome pathway
Sensitization Adverse Outcome Pathway

For more information about the ITS for Sensitization.


The In Vitro Sensitization Assay (IVSA) is a keratinocyte
activation test. IVSA uses an ELISA method to measure IL-18 release from a topically treated reconstructed 3D human
keratinocyte tissue model.


The Human Cell Line Activation Test (h-CLAT) is a dendritic
cell activation test. The h-CLAT uses flow cytometry to measure CD86 and CD54 expression on treated THP-1 cells in culture.


The Direct Peptide Reactivity Assay (DPRA) is an in chemico
method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the AOP.
The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals.

Also see: In Vitro Toxicology Testing