In Vitro Toxicology Testing with 3D Human Tissue Constructs

in vitro toxicology - tissue culture
in vitro toxicology – tissue culture
MB Research Labs – Advancing In Vitro/Alternative Test Methods.

Three dimensional human tissue constructs have proven to be very effective screening tools for irritation and corrosivity testing for the cosmetic, petrochemical, and pharmaceutical industries. Tissue constructs have been utilized for many years for ocular and dermal toxicity screening at MB Research Labs

Other tissue types (buccal, brochial, vaginal, dermal, ocular, tanned epidermis) that exhibit in vivo-like morphological and growth characteristics are available from various vendors. MB Research frequently works closely with these manufacturers to develop innovative testing protocols that fit our clients’ needs.

More and more interest in the utility of these 3D tissue models has increased especially since their use reduces the number of animals used in Toxicology testing.

MatTek EpiDerm and EpiOcular
SkinEthic Reconstituted Human Epidermal and Epithelial Models
CellSystems EpiCS® – Reconstructed Human Epidermis (RhE) models
InSphero – 3D Tissue Spheroids

Others may be available upon request.

More on In Vitro Toxicology Testing…

In Vitro Sensitization – First Steps

Sensitization testing for household and consumer products has traditionally been performed using in vivo study models with guinea pigs and mice.

MB Research now offers an Integrated Testing Strategy (ITS) of in vitro toxicology tests that follows a specific Adverse Outcome Pathway for acute dermal sensitization.

sensitization adverse outcome pathway
Sensitization Adverse Outcome Pathway

For more information about the ITS for Sensitization.


The In Vitro Sensitization Assay (IVSA) is a keratinocyte
activation test. IVSA uses an ELISA method to measure IL-18 release from a topically treated reconstructed 3D human
keratinocyte tissue model.


The Human Cell Line Activation Test (h-CLAT) is a dendritic
cell activation test. The h-CLAT uses flow cytometry to measure CD86 and CD54 expression on treated THP-1 cells in culture.


The Direct Peptide Reactivity Assay (DPRA) is an in chemico
method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the AOP.
The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals.

Also see: In Vitro Toxicology Testing