MB Research Labs – Advancing In Vitro/Alternative Test Methods.
Three dimensional human tissue constructs have proven to be very effective screening tools for irritation and corrosivity testing for the cosmetic, petrochemical, and pharmaceutical industries. Tissue constructs have been utilized for many years for ocular and dermal toxicity screening at MB Research Labs
Other tissue types (buccal, brochial, vaginal, dermal, ocular, tanned epidermis) that exhibit in vivo-like morphological and growth characteristics are available from various vendors. MB Research frequently works closely with these manufacturers to develop innovative testing protocols that fit our clients’ needs.
More and more interest in the utility of these 3D tissue models has increased especially since their use reduces the number of animals used in Toxicology testing.
The Direct Peptide Reactivity Assay (DPRA) is an in chemico
method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the AOP.
The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals.
The principle of the 3Rs was first mentioned by William Russell and Rex Burch in 1959. In their book “The Principles of Humane Experimental Technique“, they classified humane techniques under the headings Refinement, Reduction, and Replacement, commonly known now as the 3Rs.
MB Research Laboratories prides itself on pursuing in vitro and alternative toxicological methods that Reduce, Refine and Replace the use of animals in laboratory research and has worked with many leaders in this field to advance the development, validation and regulatory acceptance of in vitro and alternative tests with a goal on replacing laboratory animals in toxicological research.
MB Research is also conducting developmental and validation studies to advance application of in vitro and alternative toxicology tests. More information about currently funded research can be found here.
Data from high throughput in vitro tests are being generated for many chemicals of environmental and commercial interest, with the expectation that in vitro assay data could ultimately be used to predict adverse effects of chemical exposures in vivo. Translating values obtained from in vitro assays into estimates of in vivo outcomes is a complex process involving the use of mathematical modeling and increasingly complex test systems. This series of four webinars culminating in an in-person workshop aims to address the capabilities and the limitations of in vitro to in vivo extrapolation (IVIVE) within the context of risk decision making.
From: National Toxicology Program
Registration info here: http://ntp.niehs.nih.gov/pubhealth/evalatm/3rs-meetings/ivive-2016/ivive-2016.html
Free to the Public.
EXPERIENCE and INNOVATION.
MB Research Labs is uniquely positioned to utilize over 40 years of in vivo toxicology experience and nearly 20 years of innovating In Vitro Toxicology Testing.
Don’t Get Burned when formulating your new product.
The 3T3 NRU PT is an in vitro toxicology test used to identify the phototoxic effect of a test substance induced by the combination of test substance and light and is based on the comparison of the cytotoxic effect of a test substance when tested after the exposure and in the absence of exposure to a non-cytotoxic dose of UVA/vis light.