In Vitro Sensitization – First Steps

Sensitization testing for household and consumer products has traditionally been performed using in vivo study models with guinea pigs and mice.

MB Research now offers an Integrated Testing Strategy (ITS) of in vitro toxicology tests that follows a specific Adverse Outcome Pathway for acute dermal sensitization.

sensitization adverse outcome pathway
Sensitization Adverse Outcome Pathway

For more information about the ITS for Sensitization.

IVSA

The In Vitro Sensitization Assay (IVSA) is a keratinocyte
activation test. IVSA uses an ELISA method to measure IL-18 release from a topically treated reconstructed 3D human
keratinocyte tissue model.

h-CLAT

The Human Cell Line Activation Test (h-CLAT) is a dendritic
cell activation test. The h-CLAT uses flow cytometry to measure CD86 and CD54 expression on treated THP-1 cells in culture.

DPRA

The Direct Peptide Reactivity Assay (DPRA) is an in chemico
method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the AOP.
The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals.

Also see: In Vitro Toxicology Testing